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elucidator protein expression data analysis system software version 3.2  (Rosetta Biosoftware)

 
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    Rosetta Biosoftware elucidator protein expression data analysis system software version 3.2
    Elucidator Protein Expression Data Analysis System Software Version 3.2, supplied by Rosetta Biosoftware, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/elucidator+protein+expression+data+analysis+system+software/elucidator+protein+expression+data+analysis+system+software/pm37759504-50-3-12
    Average 90 stars, based on 1 article reviews
    elucidator protein expression data analysis system software version 3.2 - by Bioz Stars, 2026-10
    90/100 stars

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    Software:

    Article Title: dMyc expression in the fat body affects DILP2 release and increases the expression of the fat desaturase Desat1 resulting in organismal growth
    Article Snippet: MS and MS E spectra were analyzed with ProteinLynx Global Server (PLGS) software (Waters Corp.). .. In addition to PLGS software, we used the Elucidator Protein Expression Data Analysis System software (Rosetta Biosoftware). ..

    Article Title: Complement C3 interacts with cytochrome c to influence myocardial apoptosis during heart ischemia/reperfusion
    Article Snippet: .. In addition, the Elucidator Protein Expression Data Analysis System software (Rosetta Biosoftware, MA), which can import Identity E data, were used for advanced multivariate statistical analysis, false discovery control, principal components analysis, multidimensional scaling, and cluster analysis. .. To identify the C3-specific signaling network in necrosis, proteins identified from proteomics were mapped to existing biological networks by Ingenuity Pathway Analysis (QIAGEN, CA)

    Expressing:

    Article Title: dMyc expression in the fat body affects DILP2 release and increases the expression of the fat desaturase Desat1 resulting in organismal growth
    Article Snippet: MS and MS E spectra were analyzed with ProteinLynx Global Server (PLGS) software (Waters Corp.). .. In addition to PLGS software, we used the Elucidator Protein Expression Data Analysis System software (Rosetta Biosoftware). ..

    Article Title: Complement C3 interacts with cytochrome c to influence myocardial apoptosis during heart ischemia/reperfusion
    Article Snippet: .. In addition, the Elucidator Protein Expression Data Analysis System software (Rosetta Biosoftware, MA), which can import Identity E data, were used for advanced multivariate statistical analysis, false discovery control, principal components analysis, multidimensional scaling, and cluster analysis. .. To identify the C3-specific signaling network in necrosis, proteins identified from proteomics were mapped to existing biological networks by Ingenuity Pathway Analysis (QIAGEN, CA)

    Control:

    Article Title: Complement C3 interacts with cytochrome c to influence myocardial apoptosis during heart ischemia/reperfusion
    Article Snippet: .. In addition, the Elucidator Protein Expression Data Analysis System software (Rosetta Biosoftware, MA), which can import Identity E data, were used for advanced multivariate statistical analysis, false discovery control, principal components analysis, multidimensional scaling, and cluster analysis. .. To identify the C3-specific signaling network in necrosis, proteins identified from proteomics were mapped to existing biological networks by Ingenuity Pathway Analysis (QIAGEN, CA)

    other:

    Article Title: dMyc expression in the fat body affects DILP2 release and increases the expression of the fat desaturase Desat1 resulting in organismal growth
    Article Snippet: Primary rat-anti-dFOXO was used overnight at 4°C followed by Alexa-488 anti rat-secondary antibodies (Invitrogen).



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    elucidator protein expression data analysis system software version 3.2  (Rosetta Biosoftware)
    90
    Rosetta Biosoftware elucidator protein expression data analysis system software version 3.2
    Elucidator Protein Expression Data Analysis System Software Version 3.2, supplied by Rosetta Biosoftware, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/elucidator+protein+expression+data+analysis+system+software/elucidator+protein+expression+data+analysis+system+software/pm37759504-50-3-12
    Average 90 stars, based on 1 article reviews
    elucidator protein expression data analysis system software version 3.2 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    elucidator protein expression data analysis system software  (Rosetta Biosoftware)
    90
    Rosetta Biosoftware elucidator protein expression data analysis system software
    (a) Schematic diagram depicts the steps of experimental approaches. Step 1: hearts were harvested and homogenized from WT and C3 −/− mice which were subjected to 1 hr ischemia/3 hrs reperfusion. C3-containing complexes in heart lysates were immunoprecipitated with an anti-C3d Ab. Step 2: the C3-immunoprecipitated complexes were digested with trypsin and analyzed by label-free shot-gun LC-mass spectrometry. Step 3: Select detecting Ab according to proteomic information. Step 4: Sandwich ELISA to confirm the specific protein in C3-complex. (b) Left panel: Chromatograms were recorded for each biological replicate in the Resolution/Ion Mobility mode. Agglomerative hierarchical clusters of Z-score transformed intensity data were processed by the <t>Elucidator</t> program for all LC–MS chromatograms. Z-score coloration indicates protein abundance in WT compared with C3 −/− samples (red, higher abundance; green, lower abundance; black, equal abundance). Right panel histograms: Comparison of cytochrome c peptide signals of specific m/z ratios between WT and C3 −/− mice. Red and blue lines represent peptide signals from WT and C3 −/− mice, respectively. At bottom right is the summary table of the cytochrome c peptide signal comparisons of WT and C3 −/− mice. (c) Cytochrome c is present in a cytosolic C3-binding complex following 1h ischemia and 3 hrs of reperfusion. Myocardial cytosolic factions were isolated. C3-binding complexes in the myocardial cytosolic fractions were captured with a polyclonal anti-C3 Ab bound to the surface of microplates. An anti-cytochrome c Ab was used to detect cytochrome c in C3-binding complexes (n=3 mice/group). * P <0.05. Error bars indicate SEM.
    Elucidator Protein Expression Data Analysis System Software, supplied by Rosetta Biosoftware, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/elucidator+protein+expression+data+analysis+system+software/elucidator+protein+expression+data+analysis+system+software/bio_rxiv__2023__03__15__532779-36-3-10
    Average 90 stars, based on 1 article reviews
    elucidator protein expression data analysis system software - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

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    (a) Schematic diagram depicts the steps of experimental approaches. Step 1: hearts were harvested and homogenized from WT and C3 −/− mice which were subjected to 1 hr ischemia/3 hrs reperfusion. C3-containing complexes in heart lysates were immunoprecipitated with an anti-C3d Ab. Step 2: the C3-immunoprecipitated complexes were digested with trypsin and analyzed by label-free shot-gun LC-mass spectrometry. Step 3: Select detecting Ab according to proteomic information. Step 4: Sandwich ELISA to confirm the specific protein in C3-complex. (b) Left panel: Chromatograms were recorded for each biological replicate in the Resolution/Ion Mobility mode. Agglomerative hierarchical clusters of Z-score transformed intensity data were processed by the Elucidator program for all LC–MS chromatograms. Z-score coloration indicates protein abundance in WT compared with C3 −/− samples (red, higher abundance; green, lower abundance; black, equal abundance). Right panel histograms: Comparison of cytochrome c peptide signals of specific m/z ratios between WT and C3 −/− mice. Red and blue lines represent peptide signals from WT and C3 −/− mice, respectively. At bottom right is the summary table of the cytochrome c peptide signal comparisons of WT and C3 −/− mice. (c) Cytochrome c is present in a cytosolic C3-binding complex following 1h ischemia and 3 hrs of reperfusion. Myocardial cytosolic factions were isolated. C3-binding complexes in the myocardial cytosolic fractions were captured with a polyclonal anti-C3 Ab bound to the surface of microplates. An anti-cytochrome c Ab was used to detect cytochrome c in C3-binding complexes (n=3 mice/group). * P <0.05. Error bars indicate SEM.

    Journal: bioRxiv

    Article Title: Complement C3 interacts with cytochrome c to influence myocardial apoptosis during heart ischemia/reperfusion

    doi: 10.1101/2023.03.15.532779

    Figure Lengend Snippet: (a) Schematic diagram depicts the steps of experimental approaches. Step 1: hearts were harvested and homogenized from WT and C3 −/− mice which were subjected to 1 hr ischemia/3 hrs reperfusion. C3-containing complexes in heart lysates were immunoprecipitated with an anti-C3d Ab. Step 2: the C3-immunoprecipitated complexes were digested with trypsin and analyzed by label-free shot-gun LC-mass spectrometry. Step 3: Select detecting Ab according to proteomic information. Step 4: Sandwich ELISA to confirm the specific protein in C3-complex. (b) Left panel: Chromatograms were recorded for each biological replicate in the Resolution/Ion Mobility mode. Agglomerative hierarchical clusters of Z-score transformed intensity data were processed by the Elucidator program for all LC–MS chromatograms. Z-score coloration indicates protein abundance in WT compared with C3 −/− samples (red, higher abundance; green, lower abundance; black, equal abundance). Right panel histograms: Comparison of cytochrome c peptide signals of specific m/z ratios between WT and C3 −/− mice. Red and blue lines represent peptide signals from WT and C3 −/− mice, respectively. At bottom right is the summary table of the cytochrome c peptide signal comparisons of WT and C3 −/− mice. (c) Cytochrome c is present in a cytosolic C3-binding complex following 1h ischemia and 3 hrs of reperfusion. Myocardial cytosolic factions were isolated. C3-binding complexes in the myocardial cytosolic fractions were captured with a polyclonal anti-C3 Ab bound to the surface of microplates. An anti-cytochrome c Ab was used to detect cytochrome c in C3-binding complexes (n=3 mice/group). * P <0.05. Error bars indicate SEM.

    Article Snippet: In addition, the Elucidator Protein Expression Data Analysis System software (Rosetta Biosoftware, MA), which can import Identity E data, were used for advanced multivariate statistical analysis, false discovery control, principal components analysis, multidimensional scaling, and cluster analysis.

    Techniques: Immunoprecipitation, Mass Spectrometry, Sandwich ELISA, Transformation Assay, Liquid Chromatography with Mass Spectroscopy, Quantitative Proteomics, Comparison, Binding Assay, Isolation